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There's a song played for a short amount of time in Boku no Hero Academia season 3 episode 45 at 9:50. I think this song hasn't been played before in the series.

What is that song?

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The Threat of Offense and Defense (脅威との攻防 Kyōi to no kōbō)

As another user pointed out, it has actually been played at least twice before!

  • season 1 episode 10 at around 10:50
  • season 2 episode 3 when showing everyone on the minefield
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It is "Kyōi to no kōbō" (The Threat of Offence and Defense), the 29th track from Boku No Hero Academia Original Soundtrack. (YouTube link)

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